primary hsaecs Search Results


clonetics® primary human small airway epithelial cells (hsaecs)  (Cambrex)
90
Cambrex clonetics® primary human small airway epithelial cells (hsaecs)
A – C , the effect of cytochalasin D on spore internalization by A549 ( A ), HeLa ( B ) and <t>hSAECs</t> ( C ). Cells were pretreated with different concentrations (0.01 – 10 µM for A549 cells, 1 and 10 µM for HeLa and hSAECs, respectively) of cytochalasin D (Cyto D) or the solvent control (Ctrl) for 1 hr and then performed fluorescent microscopic examination as described in . Relative internalization (%) is the ratio of intracellular spores vs. total adhered spores, normalized to the solvent control. The results are the mean ± SEM from at least two independent experiments. D – F , representative images of colocalization of F-actin with spores. A549 cells were incubated with Texas-Red labeled spores and then stained with phalloidin-Alexa Fluor 488. G , colocalization of F-actin with spores is inhibited by Cyto D. Cyto D (10 µM) was included in the colocalization examination as described in . The percentage of spores with enriched F-actin staining vs. total attached spores is denoted as %Colocalization. The results shown are combined from five independent experiments. ***, p< 0.0001, t test. Scale bars represent 5 µm. H – J , Cdc42 is the primary Rho-family GTPase required for spore uptake. A549 cells were transfected with pcDNA3.1(+) vector control (Ctrl), HA-T19NRhoA (T19NRhoA), HA-T17NRac1 (T17NRac1) or HA-T17NCdc42 (T17NCdc42). The expression of the transfected constructs was verified using western blot analysis 24 hr post transfection ( H ). Dominant negative GTPases were detected by anti-HA antibodies and secondary antibodies as described in . The actin level was used as a loading control. Spore uptake by ( I ) and adherence to ( J ) transfected cells were determined by gentamicin protection assays 24 hr post transfection as described in . Relative internalization (%) is the ratio of intracellular spores vs. total spores added, normalized to the solvent control. Relative association (%) is the ratio of associated spores (extracellular adhered + intracellular) vs. total spores added, normalized to the solvent control. The results shown are the mean ± SEM, combined from three independent experiments. **, p = 0.0018, t test.
Clonetics® Primary Human Small Airway Epithelial Cells (Hsaecs), supplied by Cambrex, used in various techniques. Bioz Stars score: 90/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
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Average 90 stars, based on 1 article reviews
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primary hsaecs  (Lifeline Cell Technology)
90
Lifeline Cell Technology primary hsaecs
A – C , the effect of cytochalasin D on spore internalization by A549 ( A ), HeLa ( B ) and <t>hSAECs</t> ( C ). Cells were pretreated with different concentrations (0.01 – 10 µM for A549 cells, 1 and 10 µM for HeLa and hSAECs, respectively) of cytochalasin D (Cyto D) or the solvent control (Ctrl) for 1 hr and then performed fluorescent microscopic examination as described in . Relative internalization (%) is the ratio of intracellular spores vs. total adhered spores, normalized to the solvent control. The results are the mean ± SEM from at least two independent experiments. D – F , representative images of colocalization of F-actin with spores. A549 cells were incubated with Texas-Red labeled spores and then stained with phalloidin-Alexa Fluor 488. G , colocalization of F-actin with spores is inhibited by Cyto D. Cyto D (10 µM) was included in the colocalization examination as described in . The percentage of spores with enriched F-actin staining vs. total attached spores is denoted as %Colocalization. The results shown are combined from five independent experiments. ***, p< 0.0001, t test. Scale bars represent 5 µm. H – J , Cdc42 is the primary Rho-family GTPase required for spore uptake. A549 cells were transfected with pcDNA3.1(+) vector control (Ctrl), HA-T19NRhoA (T19NRhoA), HA-T17NRac1 (T17NRac1) or HA-T17NCdc42 (T17NCdc42). The expression of the transfected constructs was verified using western blot analysis 24 hr post transfection ( H ). Dominant negative GTPases were detected by anti-HA antibodies and secondary antibodies as described in . The actin level was used as a loading control. Spore uptake by ( I ) and adherence to ( J ) transfected cells were determined by gentamicin protection assays 24 hr post transfection as described in . Relative internalization (%) is the ratio of intracellular spores vs. total spores added, normalized to the solvent control. Relative association (%) is the ratio of associated spores (extracellular adhered + intracellular) vs. total spores added, normalized to the solvent control. The results shown are the mean ± SEM, combined from three independent experiments. **, p = 0.0018, t test.
Primary Hsaecs, supplied by Lifeline Cell Technology, used in various techniques. Bioz Stars score: 90/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
https://www.bioz.com/result/primary hsaecs/product/Lifeline Cell Technology
Average 90 stars, based on 1 article reviews
primary hsaecs - by Bioz Stars, 2026-02
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primary human small airway epithelial cells hsaec  (LifeCell Inc)
90
LifeCell Inc primary human small airway epithelial cells hsaec
A – C , the effect of cytochalasin D on spore internalization by A549 ( A ), HeLa ( B ) and <t>hSAECs</t> ( C ). Cells were pretreated with different concentrations (0.01 – 10 µM for A549 cells, 1 and 10 µM for HeLa and hSAECs, respectively) of cytochalasin D (Cyto D) or the solvent control (Ctrl) for 1 hr and then performed fluorescent microscopic examination as described in . Relative internalization (%) is the ratio of intracellular spores vs. total adhered spores, normalized to the solvent control. The results are the mean ± SEM from at least two independent experiments. D – F , representative images of colocalization of F-actin with spores. A549 cells were incubated with Texas-Red labeled spores and then stained with phalloidin-Alexa Fluor 488. G , colocalization of F-actin with spores is inhibited by Cyto D. Cyto D (10 µM) was included in the colocalization examination as described in . The percentage of spores with enriched F-actin staining vs. total attached spores is denoted as %Colocalization. The results shown are combined from five independent experiments. ***, p< 0.0001, t test. Scale bars represent 5 µm. H – J , Cdc42 is the primary Rho-family GTPase required for spore uptake. A549 cells were transfected with pcDNA3.1(+) vector control (Ctrl), HA-T19NRhoA (T19NRhoA), HA-T17NRac1 (T17NRac1) or HA-T17NCdc42 (T17NCdc42). The expression of the transfected constructs was verified using western blot analysis 24 hr post transfection ( H ). Dominant negative GTPases were detected by anti-HA antibodies and secondary antibodies as described in . The actin level was used as a loading control. Spore uptake by ( I ) and adherence to ( J ) transfected cells were determined by gentamicin protection assays 24 hr post transfection as described in . Relative internalization (%) is the ratio of intracellular spores vs. total spores added, normalized to the solvent control. Relative association (%) is the ratio of associated spores (extracellular adhered + intracellular) vs. total spores added, normalized to the solvent control. The results shown are the mean ± SEM, combined from three independent experiments. **, p = 0.0018, t test.
Primary Human Small Airway Epithelial Cells Hsaec, supplied by LifeCell Inc, used in various techniques. Bioz Stars score: 90/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
https://www.bioz.com/result/primary human small airway epithelial cells hsaec/product/LifeCell Inc
Average 90 stars, based on 1 article reviews
primary human small airway epithelial cells hsaec - by Bioz Stars, 2026-02
90/100 stars
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Image Search Results


A – C , the effect of cytochalasin D on spore internalization by A549 ( A ), HeLa ( B ) and hSAECs ( C ). Cells were pretreated with different concentrations (0.01 – 10 µM for A549 cells, 1 and 10 µM for HeLa and hSAECs, respectively) of cytochalasin D (Cyto D) or the solvent control (Ctrl) for 1 hr and then performed fluorescent microscopic examination as described in . Relative internalization (%) is the ratio of intracellular spores vs. total adhered spores, normalized to the solvent control. The results are the mean ± SEM from at least two independent experiments. D – F , representative images of colocalization of F-actin with spores. A549 cells were incubated with Texas-Red labeled spores and then stained with phalloidin-Alexa Fluor 488. G , colocalization of F-actin with spores is inhibited by Cyto D. Cyto D (10 µM) was included in the colocalization examination as described in . The percentage of spores with enriched F-actin staining vs. total attached spores is denoted as %Colocalization. The results shown are combined from five independent experiments. ***, p< 0.0001, t test. Scale bars represent 5 µm. H – J , Cdc42 is the primary Rho-family GTPase required for spore uptake. A549 cells were transfected with pcDNA3.1(+) vector control (Ctrl), HA-T19NRhoA (T19NRhoA), HA-T17NRac1 (T17NRac1) or HA-T17NCdc42 (T17NCdc42). The expression of the transfected constructs was verified using western blot analysis 24 hr post transfection ( H ). Dominant negative GTPases were detected by anti-HA antibodies and secondary antibodies as described in . The actin level was used as a loading control. Spore uptake by ( I ) and adherence to ( J ) transfected cells were determined by gentamicin protection assays 24 hr post transfection as described in . Relative internalization (%) is the ratio of intracellular spores vs. total spores added, normalized to the solvent control. Relative association (%) is the ratio of associated spores (extracellular adhered + intracellular) vs. total spores added, normalized to the solvent control. The results shown are the mean ± SEM, combined from three independent experiments. **, p = 0.0018, t test.

Journal: PLoS ONE

Article Title: Bacillus anthracis Spore Entry into Epithelial Cells Is an Actin-Dependent Process Requiring c-Src and PI3K

doi: 10.1371/journal.pone.0011665

Figure Lengend Snippet: A – C , the effect of cytochalasin D on spore internalization by A549 ( A ), HeLa ( B ) and hSAECs ( C ). Cells were pretreated with different concentrations (0.01 – 10 µM for A549 cells, 1 and 10 µM for HeLa and hSAECs, respectively) of cytochalasin D (Cyto D) or the solvent control (Ctrl) for 1 hr and then performed fluorescent microscopic examination as described in . Relative internalization (%) is the ratio of intracellular spores vs. total adhered spores, normalized to the solvent control. The results are the mean ± SEM from at least two independent experiments. D – F , representative images of colocalization of F-actin with spores. A549 cells were incubated with Texas-Red labeled spores and then stained with phalloidin-Alexa Fluor 488. G , colocalization of F-actin with spores is inhibited by Cyto D. Cyto D (10 µM) was included in the colocalization examination as described in . The percentage of spores with enriched F-actin staining vs. total attached spores is denoted as %Colocalization. The results shown are combined from five independent experiments. ***, p< 0.0001, t test. Scale bars represent 5 µm. H – J , Cdc42 is the primary Rho-family GTPase required for spore uptake. A549 cells were transfected with pcDNA3.1(+) vector control (Ctrl), HA-T19NRhoA (T19NRhoA), HA-T17NRac1 (T17NRac1) or HA-T17NCdc42 (T17NCdc42). The expression of the transfected constructs was verified using western blot analysis 24 hr post transfection ( H ). Dominant negative GTPases were detected by anti-HA antibodies and secondary antibodies as described in . The actin level was used as a loading control. Spore uptake by ( I ) and adherence to ( J ) transfected cells were determined by gentamicin protection assays 24 hr post transfection as described in . Relative internalization (%) is the ratio of intracellular spores vs. total spores added, normalized to the solvent control. Relative association (%) is the ratio of associated spores (extracellular adhered + intracellular) vs. total spores added, normalized to the solvent control. The results shown are the mean ± SEM, combined from three independent experiments. **, p = 0.0018, t test.

Article Snippet: Clonetics® primary human small airway epithelial cells (hSAECs) (Cambrex) were maintained at 37°C in a humidified chamber with 5% CO 2 in SABM media supplemented with reagents from the SAGM SingleQuot kit (Cambrex), following instructions from the supplier.

Techniques: Solvent, Control, Incubation, Labeling, Staining, Transfection, Plasmid Preparation, Expressing, Construct, Western Blot, Dominant Negative Mutation